ASCP CG Certification Practice Exam Questions and Answers ASCP CG Certification Practice Exam Questions and Answers 1. What is the best agent to use against mycoplasma?: BM cyclin - a combina- tion of two antibiotics (tiamulin and minocycline) that effecticely targets mycoplas- ma growth 2. For successful harvest, each new batch of hypotonic solution should be evaluated by:: comparing the new lot to a lot already in use 3. Colagenase, pronase, and trypsin are:: Proteolytic enzymes - break down tissues into singlecell suspensions 4.When harvesting peripheral blood cultures, asceptic technique no longer matters following incubation with:: Colcemid - mitotic spindle poison that halts cells at metaphase 5. Samplesthat arrive at the cytogeneticslab should first be:: Logged in to the system 6. Phytohemagglutinin (PHA) stimulates which type of cells into division?: - T-cell lymphocytes 7. To avoid culture failure, monitoring reagents is crucial.When logging a culture failure, what is the MOST important to document?: Expiration dates of reagents used 8. Which type of microscope is used when cleaning a CVS (chorionic villus) sample?: Dissection 9. Which cell type is most problematic if found in a CVS (chorionic villus) sample?: Decidua (maternal tissue) 10. When looking at a slide under a phase contrast scope and a lot of cytoplasm is still seen around the mets, what is the most likely cause?: in- nappropriate slide drying time 11. What will help prevent microbial contamination and cross contamination of cultures?: Asceptic technique 12. When there are not enough mets to complete the study of a patient/case, what needs to be done next?: prepare additional slides from any remaining sample 13. When looking at a dropped slide under a phase scope and mets are very spread out and sparse, what could help fix the issue?: Increading the drying time by using warm slides 14. What is true of an inverted microscope?: the objectives are beneath the stage 15. High-resolution banding is indicated for a possible diagnosis of:: - DiGenorge syndrome -- microdeletion of 22q11, also called velocardiofacial syn- drome "velo" - soft palette, "cardio" - congenital heart defects, "facial" - unique abnormal facial features, short philtrum (upper lip indent), tubular nose, wide spread eyes, etc 16.When verifying if a specimen is appropriate for cytogenetic analysis,what is the MOST important data to accompany the specimen?: Reason for referral 17. Which type of specimen should be requested for a cytogenetic analysis for turner syndrome?: Heparinized peripheral blood stored at room temperature
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